Hot Start

TEMPase Hot Start DNA Polymerase

TEMPase Hot Start DNA Polymerase has been designed to diminish the formation of non-specific priming events during reaction set-up and the first ramp of thermal cycling. TEMPase Hot Start DNA Polymerase therefore enables detection of low abundance targets as well as multiplexing purposes. 


FEATURES

  • Reaction set-up at room temperature
  • dUTP incorporation possible
  • Increased sensitivity, specificity and product yield


DESCRIPTION

TEMPase Hot Start DNA Polymerase is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment.

A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature.
During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase. Resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase.

TEMPase Hot Start DNA Polymerase is suitable for detection of low abundance targets, screening, multiplexing, direct colony PCR and Real time PCR applications.

 

TEMPASE HOT START DNA POLYMERASE PROMOTES INCREASED SPECIFICITY AND YIELD

Example of PCR amplification of BAIP3. Taq or TEMPase were used as indicated with Ammonium Buffer at the indicated Mg2+ concentrations. Taq results in specific and high yield band at only one Mg2+ concentration (2 mM). TEMPase results in specific bands over a broad range of Mg2+ concentrations and increased yield. M: Marker.

 

TEMPASE HOT START DNA POLYMERASE IS INACTIVE AT AMBIENT TEMPERATURE

 

 

 


Ampliqon TEMPase is activated by initial heating at 95 °C for 15 minutes. (lane 1). Without activation the enzyme is completely inactive (lane 2). M: marker.

Product info

TEMPase Hot Start DNA Polymerase

With Ammonium Buffer
With Combination Buffer
5x PCR Buffer RED
Without buffer
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Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com