Hot Start

TEMPase Hot Start Master Mix

TEMPase Hot Start Master Mix is a ready to use mix that offers easy reaction assembly at room temperature prior to hot start PCR. Just add your template and primers to successfully carry out PCR.
TEMPase Hot Start Master Mix is the popular alternative to TEMPase Hot Start DNA Polymerase. It offers the same excellent performance and increased reproducibility.


FEATURES

  • Minimal optimisation
  • Convenient reaction set up at room temperature
  • Time-saving premixed solution
  • Increased sensitivity, specificity and product yield
  • Consistent test results
  • Lower risk of contamination

 
DESCRIPTION

Ampliqon TEMPase Hot Start DNA Polymerase 2x Master Mix is a ready to use master mix composed of TEMPase Hot Start DNA Polymerase, dNTPs, MgCland either TEMPase Buffer C (a balanced KCI/(NH4)2 SO4 Tris buffer system) or TEMPase Buffer A (a (NH4)2 SO4 tris buffer system).

The TEMPase buffer system covers all needs for PCR applications and allow the user to choose the optimal buffer for specific PCR set-ups and applications.

TIP - CHOOSE THE RIGHT TEMPASE HOT START MASTER MIX

For most standard applications Master Mix A based on Ammonium Buffer is the best option. It promotes robust amplification, high yield and high specificity. In certain cases, Master Mix C is the best option.

If you want to visualise on agarose gels, we suggest that you choose Master Mix BLUE A or master Mix BLUE C.

 

COMPARISON OF TEMPASE HOT START POLYMERASE 2X MASTER MIX WITH COMPETITOR

TEMPase Hot Start DNA Polymerase 2x Master Mix A was compared with an equivalent Hot Start master mix from a well-recognised competitor. Four different DNA targets varying in length PAH (203 bp), ENG9 (293 bp), Q8 (727 bp) and ENG5 (480 bp) were evaluated. TEMPase Hot Start DNA Polymerase 2x Master Mix A performed equally well or better than competitor. Each Amplification has been conducted according to supplier's manual.

Product info

TEMPase Hot Start Master Mix

Master Mix A
Master Mix C

 

Need help choosing a PCR enzyme product, getting prices, placing an order or tracking an order?

Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com