Multiplex

Multiplex TEMPase Master Mix

Multiplex TEMPase Master Mix is developed for the simultaneous amplification of two or more amplicons in a single tube. Multiplex TEMPase Master Mix minimises the need for optimisation and makes the development of multiplex PCR fast and easy.


FEATURES

  • Amplification of multiple PCR products in one tube
  • Diminished formation of non-specific products
  • Detection of low abundance targets
  • Reaction set-up at room temperature
  • High sensitivity, sensitivity and product yield


DESCRIPTION

Multiplex 2x master mix is composed of TEMPase Hot Start DNA Polymerase and a specialised buffer system designed for multiplex PCR. TEMPase Hot Start DNA Polymerase is well suited for multiplex PCR due to its high specificity. MgCl2 is enclosed in the multiplex kit to enable optimisation. 

 

AMPLIFICATION OF A FIVE-PLEX AND A TEN-PLEX REACTION

Five different templates of the CFTR gene (5-plex) and ten different templates of the DMD gene (10-plex) were amplified simultaneously in one tube, respectively. M: Marker.

Product info

Multiplex TEMPase Master Mix

2x Master Mix, 3 mM MgCl2 final

 

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