5x PCR Buffer RED is a ready to use PCR buffer, including all components for standard PCR applications.
FEATURES
All in one Buffer for highest convenience
Direct gel loading onto agarose gels
Red colour enables easy visualisation of pipetting and loading
Dye front runs at 1000 - 2000 bp on DNA 0.5 - 1.5 % agarose
Same specifications as Ammonium Buffer
5x Buffer
DESCRIPTION
5x PCR Buffer RED offers the same specification as Ammonium Buffer. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures. The included red dye and density reagent, eliminates the need for loading dye as well as the time consuming sample preparation before electrophoresis.
DIRECT GEL LOADING
After end of amplification PCR products can be loaded directly onto agarose and SDS DNA gels. The red dye provides easy visualisation of both pipetting and gel loading. The red dye front runs at 1000 - 2000 bp on 0.5 - 1.5 % agarose gels.
5X PCR BUFFER RED PROMOTES SPECIFIC AND CLEAR RESULTS
5x PCR Buffer RED promotes specific and clear results. The same DNA target was evaluated using the same PCR set-up in duplicates amplified by either Ampliqon Taq DNA Polymerase or TEMPase Hot Start DNA Polymerase.
