PCR Buffers

Combination Buffer

Combination Buffer is a balanced ammonium-potassium PCR buffer. Combination Buffer results in tolerance towards optimization of primer annealing temperatures and Mg2+ concentrations.


FEATURES

  • Promotes reliable amplification on most DNA templates
  • Tolerance toward primer annealing temperature
  • 10x Buffer


AVAILABLE IN 4 FORMULATIONS:

  • 15 mM MgCl2,
  • Mg2+ free
  • Tween free
  • Mg2+ + Tween free


DESCRIPTION

Ammonium Buffer is recommended for most PCR applications however; we recommend you to use Combination Buffer as it show good results on certain PCR instruments. Combination Buffer is also worth testing when searching for PCR buffers to be used in new set-up.


TIP - CHOOSE THE RIGHT BUFFER

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.

Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

 

PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM

Ammonium Buffer: Minimal need for optimisation. 
A broad range of Mg2+ concentrations and temperatures results in specific products with high yield (Lanes 1.5 - 2.5 and lanes 57-66)


Standard Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products. (Lanes 1.5 - 2.0 and lanes 60-66)


Combination Buffer: 
Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg2+ concentrations or temperatures. The first part shows a Mg2+ dilution series from 0.5 - 4.5 mM MgCl2, at 60°C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl2. M: marker.

Product info

Combination Buffer

15 mM MgCl2
Mg2+ free
Tween free
Mg2+ free, Tween free

 

Need help choosing a PCR enzyme product, getting prices, placing an order or tracking an order?

Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com