10x Standard Buffer

10x Standard Buffer is the traditional potassium buffer and promotes high specificity.

FEATURES

• Promotes reliable amplification on most DNA templates
• High specificity
• 10x Buffer

AVAILABLE IN 4 FORMULATIONS:

• 15 mM MgCl_2,
• Mg²⁺ free
• Tween free
• Mg²⁺ free + Tween free

DESCRIPTION

10x Ammonium Buffer is recommended for most PCR applications however; we recommend that you continue using 10x Standard Buffer if you have already optimised your protocols for this buffer. The use of 10x Standard buffer most often requires optimisation of primer annealing temperatures and Mg²⁺ concentrations. Highly pure DNA templates are preferable if you use this buffer.

TIP - CHOOSE THE RIGHT BUFFER

10x Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg²⁺ concentrations or the annealing temperatures.

Mg²⁺ free buffer is recommended if you need to optimise Mg²⁺ concentrations in your PCR set-up, especially if your application requires Mg²⁺ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM

Ammonium Buffer: Minimal need for optimisation.
A broad range of Mg²⁺ concentrations and temperatures results in specific products with high yield (Lanes 1.5 - 2.5 and lanes 57-66)

Standard Buffer: Optimisation is needed.
A narrow range of Mg²⁺ concentrations and temperatures results in specific products. (Lanes 1.5 - 2.0 and lanes 60-66)

Combination Buffer: Optimisation is needed.
A narrow range of Mg²⁺ concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg²⁺ concentrations or temperatures. The first part shows a Mg²⁺ dilution series from 0.5 - 4.5 mM MgCl₂, at 60°C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl₂. M: marker.