PCR Buffers

Standard Buffer

Standard Buffer is the traditional potassium buffer and promotes high specificity.


FEATURES

  • Promotes reliable amplification on most DNA templates
  • High specificity
  • 10x Buffer


AVAILABLE IN 4 FORMULATIONS:

  • 15 mM MgCl2,
  • Mg2+ free
  • Triton-X 100 free
  • Mg2++ Triton-X 100 free


DESCRIPTION

Ammonium Buffer is recommended for most PCR applications however; we recommend that you continue using Standard Buffer if you have already optimised your protocols for this buffer. The use of Standard buffer most often requires optimisation of primer annealing temperatures and Mg2+ concentrations. Highly pure DNA templates are preferable if you use this buffer.

TIP - CHOOSE THE RIGHT BUFFER

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.

Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

 

PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM

Ammonium Buffer: Minimal need for optimisation.
A broad range of Mg2+ concentrations and temperatures results in specific products with high yield (Lanes 1.5 - 2.5 and lanes 57-66)


Standard Buffer: 
Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products. (Lanes 1.5 - 2.0 and lanes 60-66)


Combination Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg2+ concentrations or temperatures. The first part shows a Mg2+ dilution series from 0.5 - 4.5 mM MgCl2, at 60°C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl2. M: marker.

Product info

Standard Buffer

15 mM MgCl2
Mg2+ free
Triton free
Mg2+ free, Triton free

 

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Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com