Taq

Taq DNA Polymerase Master Mix RED

Taq DNA Polymerase Master Mix RED is a very popular alternative to Taq DNA Polymerase Master Mix with the same advantages. The inert red dye and stabiliser are extra features, which allow direct loading onto your agarose gel for analysis.


FEATURES

  • Premixed solution
  • Minimal optimisation
  • Direct loading onto agarose and SDS DNA gels
  • Easy visualisation of pipetting
  • Dye front runs at 300 - 1000 bp on 0.5 - 1.5 % agarose gel
  • No need for a separate loading dye
  • Increased reproducibility
  • High product yield
  • dUTP incorporation possible
  • Processes up to 5 kb
  • Leaves a 3'dA overhang


DESCRIPTION

Taq DNA Polymerase Master Mix RED is a ready to use 1.1x or 2x master mix. Taq DNA Polymerase Master Mix RED is available in two final MgClconcentrations: 1.5 mM and 2.0 mM. Taq DNA Polymerase Master Mix RED is composed of Ampliqon Taq DNA Polymerase, the NH4+ buffer system, dNTPs and magnesium chloride.

The red dye and the stabiliser do not interfere with the PCR. If necessary, the red dye can be removed from the PCR product by spin column purification or other methods. Furthermore, PCR products generated using Taq DNA Polymerase Master Mix RED DNA, can directly be used for sequencing after treatment with either spin columns or ExoSAP-IT. For information about the ExoSAP-IT protocol please contact us.

DIRECT GEL LOADING

After end of amplification PCR products can be loaded directly onto agarose and SDS DNA gels. The red dye provides easy visualisation of both pipetting and gel loading. The red dye front runs at 300 - 1000 bp on 0.5 - 1.5 % agarose gels.

 

COMPARISON BETWEEN TAQ DNA POLYMERASE MASTER MIX RED AND TWO COMPETITORS

Ampliqon Taq DNA Polymerase Master Mix RED was compared with a Taq DNA Polymerase master mixes from two well recognised competitors. Four different DNA target varying in length PAH (203 bp), Q8 (727 bp), CFTR (613 bp) and BAIP (788) were evaluated. Ampliqon Taq DNA Polymerase Master Mix RED performed equally well or better in this study. Each Amplification has been conducted according to suppliers manual.

 

RED DYE EXHIBITS NO PCR INTERFERENCE

The red dye does not interfere with PCR performance. Two different DNA targets; PAH (203 bp) and BAIP (788 bp) were amplified in duplicates using Taq Master Mix RED and visualised on a agarose gel.

Product info

Taq DNA Polymerase Master Mix RED

1.1x and 1.5 MgCl2
1.1x and 2.0 mM MgCl2
2x and 1.5 mM MgCl2
2x and 2.0 mM MgCl2

 

Need help choosing a PCR enzyme product, getting prices, placing an order or tracking an order?

Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com