Taq DNA Polymerase Glycerol Free 5 U/µl is a glycerol free formulation of our regular Taq DNA Polymerase and is well suited for automated high throughput testing, lyophilization or where accurate pipetting of small volumes are crucial. Taq DNA Polymerase Glycerol Free 5 U/µl is available in different sizes and buffer formats.
FEATURES
Glycerol free storage buffer
Suitable for automated high throughput testing
Suitable for applications involving freeze drying
Suitable for robot-aided pipetting
High product yield
Processes up to 5 kb
dUTP incorporation possible
Leaves a 3'dA overhang
DESCRIPTION
Ampliqon Taq DNA Polymerase Glycerol Free 5 U/µl exhibits both a 5'→3'dA DNA polymerase and a 5'→3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability.
WHAT GLYCEROL DOES
Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
TAQ DNA POLYMERASE GLYCEROL FREE FOR AUTOMATION AND FREEZE DRYING
Ampliqon Taq DNA Polymerase Glycerol Free is developed for applications involving automation and freeze drying. Its Glycerol free formulation is well suited for automated routine PCR applications, or where accurate pipetting of small volumes is crucial.
