AQ90 High Fidelity DNA Polymerase 2x Master Mix is a convenient alternative to AQ90 High Fidelity DNA Polymerase, displaying the following features; high fidelity - measured up to 50x Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets.
AQ90 High Fidelity DNA Polymerase 2x Master Mix is recommended for applications, which require extremely high fidelity such as cloning/Sub-cloning, NGS applications and mutagenesis.
AQ90 High Fidelity DNA Polymerase 2x Master Mix is a ready to use 2x reaction mix composed of AQ90 High Fidelity DNA Polymerase and an optimised buffer system including dNTPs and magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets.
AQ90 High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.
For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.
The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerases error rate. Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.
For more information about fidelity read here.
AQ90 2X MASTER MIX PROMOTES ROBUST AMPLIFICATION
LONG RANGE AMPLIFICATION