TEMPase Hot Start DNA Polymerase 5 U/µl has been designed to diminish the formation of non-specific priming events during reaction set-up and the first ramp of thermal cycling. TEMPase Hot Start DNA Polymerase therefore enables detection of low abundance targets as well as multiplexing purposes.
FEATURES
Reaction set-up at room temperature
dUTP incorporation possible
Increased sensitivity, specificity and product yield
DESCRIPTION
TEMPase Hot Start DNA Polymerase is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature. During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase. Resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase.
TEMPase Hot Start DNA Polymerase is suitable for detection of low abundance targets, screening, multiplexing, direct colony PCR and Real time PCR applications.
TEMPASE HOT START DNA POLYMERASE PROMOTES INCREASED SPECIFICITY AND YIELD
Example of PCR amplification of BAIP3. Taq or TEMPase Hot Start DNA Polymerases were used for amplification of BAIP3 with Ammonium Buffer and the indicated Mg2+ concentrations. TEMPase Host Start DNA Polymerase provides specific amplification over a broad range of Mg2+ concentrations and increased yield. In contrary, Taq DNA Polymerase only provides specific amplification at 2mM Mg2+. TM: Marker.
TEMPASE HOT START DNA POLYMERASE IS INACTIVE AT AMBIENT TEMPERATURE
TEMPase Hot Start DNA Polymerase is activated by initial heating at 95 °C for 15 minutes. (lane 1). Without activation the enzyme is completely inactive (lane 2). M: marker.
