Hot Start

TEMPase Hot Start Master Mix BLUE

TEMPase Hot Start 2x Master Mix BLUE is a time saving alternative to TEMPase Hot Start 2x Master Mix. It offers the same excellent performance. In addition, the blue loading dye facilitates direct gel loading. You do not need a separate loading buffer and time-consuming sample preparation before electrophoresis. This makes TEMPase Hot Start Master Mix BLUE especially suitable for high throughput testing.


  • Minimal optimisation
  • Time-saving premixed solution
  • Direct loading onto agarose and SDS DNA gels
  • Easy visualisation of pipetting
  • Dye front running at 100-500 bp (0.5 -1.5 % agarose gel)
  • Convenient reaction set-up at room temperature
  • Increased sensitivity, specificity and product yield
  • Consistent test results
  • Lower risk of contamination


TEMPase Hot Start DNA Polymerase Master Mix BLUE is a ready-to-use 2x master mix composed of dNTPs, a blue loading dye, a stabiliser, MgCl2 and either TEMPase Buffer C (a balanced KCl / (NH4)2 SO4 buffer system)  or TEMPase Buffer A (a (NH4)2 SO4 buffer system).

The blue dye and stabiliser do not interfere with the PCR. If necessary, the blue dye can be removed by spin column purification or other methods. Furthermore, PCR products generated using TEMPase Hot Start DNA Master Mix BLUE, can directly be used for sequencing after treatment with either spin columns or PureIT ExoZAP PCR CleanUp. 



For most standard applications Master Mix BLUE A based on Ammonium Buffer is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix BLUE C is the best option.



After end of amplification, using TEMPase DNA Polymerase Hot Start Master Mix BLUE PCR products can be loaded directly onto agarose and SDS DNA gels. The blue dye provides easy visualisation of both pipetting and gel loading. The blue dye front runs at 100 - 500 bp on 0.5 - 1.5 % agarose gels.

Product info

TEMPase Hot Start Master Mix BLUE

Master Mix A
Master Mix C


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