TEMPase Hot Start DNA Polymerase 2x Master Mix is a ready to use mix offering easy reaction assembly at room temperature prior to PCR. Just add your template and primers to successfully carry out PCR. TEMPase Hot Start DNA Polymerase 2x Master Mix is the popular alternative to TEMPase Hot Start DNA Polymerase. It offers the same excellent performance and increased reproducibility.
FEATURES
Minimal optimisation
Convenient reaction set up at room temperature
Time-saving premixed solution
Increased sensitivity, specificity and product yield
Consistent test results
Lower risk of contamination
DESCRIPTION
Ampliqon TEMPase Hot Start DNA Polymerase 2x Master Mix is a ready to use master mix composed of TEMPase Hot Start DNA Polymerase, dNTPs, MgCl2 and either TEMPase Buffer C (a balanced KCI/(NH4)2 SO4 Tris buffer system) or TEMPase Buffer A (a (NH4)2 SO4 tris buffer system).
The TEMPase buffer system covers all needs for PCR applications and allow the user to choose the optimal buffer for specific PCR set-ups and applications.
TIP - CHOOSE THE RIGHT TEMPASE HOT START MASTER MIX
For most standard applications 2x Master Mix A based on Ammonium Buffer is the best option. It promotes robust amplification, high yield and high specificity. In certain cases, 2x Master Mix C is the best option.
If you want to visualise on agarose gels, we suggest that you choose 2x Master Mix BLUE A or 2x Master Mix BLUE C.
PERFORMANCE OF TEMPASE HOT START DNA POLYMERASE 2X MASTER MIX A
TEMPase Hot Start DNA Polymerase 2x Master Mix A was compared with an equivalent Hot Start 2x master mix from a well-recognised competitor. Four different DNA targets varying in length PAH (203 bp), ENG9 (293 bp), Q8 (727 bp) and ENG5 (480 bp) were evaluated. TEMPase Hot Start DNA Polymerase 2x Master Mix A performed equally well or better than competitor.
Each Amplification has been conducted according to supplier's manual.
