Lyophilized DryTech TEMPase 5x Master Mix Clear for shipping and storage at ambient temperature. Fewer handling steps lead to better reproducibility, which makes DryTech TEMPase 5x Master Mix Clear suitable for standard PCR applications, screening and high throughput testing. The included 5x DryTech TEMPase 5x Master Mix Clear is easily reconstituted in the 5x DryTech Buffer Clear, provided with the kit.
FEATURES
Lyophilized PCR master mix
Easily reconstituted in 5x DryTech Buffer Clear
Shipping and storage at ambient temperature
Cost saving shipment
Stable for 12 months ≤ 25 ºC
All-in-one 5x PCR master mix for great convenience
Processes up to 4 kb
Hot start technology
DESCRIPTION
DryTech TEMPase Master Mix Clear is a lyophilized and optimized version of the well-established liquidized TEMPase Hot Start DNA Polymerase Master Mix from Ampliqon. The lyophilized format allows shipment and storage without cooling. Shipment without cooling reduces both transportation cost and the impact on CO2.
DryTech TEMPase 5x Master Mix Clear is composed of Ampliqon TEMPase Hot Start DNA Polymerase, the NH4+ buffer system, dNTPs and magnesium chloride.
The reconstituted DryTech TEMPase 5x Master Mix Clear can be stored at 4 °C for 3 months and at -20 °C for prolonged stability up to 6 months.
COMPETITOR COMPARISON – LYOPHILIZED MASTER MIXES
Ampliqon DryTech TEMPase Master Mix Clear was compared to a lyophilized master mix from a well-recognized competitor. Five different DNA targets varying in lengths were evaluated. Ampliqon DryTech TEMPase Master Mix performed equally well in this study. Each amplification has been conducted according to manufactures manuals. Lane 1 +6: PAH (203 bp), Lane 2+7: CFTR (613 bp), Lane 3+8: BAIP (788 bp), Lane 4+9: PAH1963 (1963 bp) and Lane 5: CFTR3053 (3053 bp). M: Marker (Iqon PCR Ladder).
