PCR Clean-up for optimal sequencing results. Clean-up results in 5 minutes.
PureIT ExoZAP PCR CleanUp Kit consists of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP).
Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp Kit, degrades residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp Kit directly to the reaction containing the amplified PCR product. After treatment at 37 °C for minimum 2 minutes both enzymes are completely inactivated by heating at 80 °C for minimum 3 minutes.
This protocol serves as a guideline for clean-up of 5 μl PCR product using PureIT ExoZAP PCR CleanUp Kit*.
*If treating PCR product of higher volume, then increase proportionally the amount of HL-ExoI and rSAP.
**The enzymes within PureIT ExoZAP PCR CleanUp Kit works in PCR buffers.
PureIT ExoZAP PCR CleanUp IMPROVES QUALITY OF DNA SEQUENCING