Amplification of DNA with a high percentage of GC content is often compromised by inadequate strand separation and complex secondary structure formations. The inability of the DNA polymerase to read through strong secondary structures such as hairpins, results in very low PCR yield and formation of truncated PCR products.
Ampliqon has developed a unique solution for amplification of GC-rich and other difficult DNA targets.
GC Buffer I or GC Buffer II in combination with TEMPase Hot Start DNA polymerase promotes excellent amplification results of DNA targets with varying high degrees of GC content.
GC TEMPase master mixes, GC Buffer I and GC Buffer II are manufactured in-house in accordance to our ISO 9001:2008 quality management system, ensuring that each batch possess the same robust performance every time.