Taq DNA Polymerase Glycerol Free is a glycerol free formulation of our regular Taq DNA Polymerase and is well suited for automated high throughput testing, lyophilization or where accurate pipetting of small volumes are crucial. Taq DNA Polymerase Glycerol Free is available in different sizes and two formats; 5 U/µl and as highly concentrated 50 U/µl.
Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5'→3'dA DNA polymerase and a 5'→3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability.
WHAT GLYCEROL DOES
Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
TAQ DNA POLYMERASE GLYCEROL FREE FOR AUTOMATION AND FREEZE DRYING
Taq DNA Polymerase Glycerol Free