High Fidelity

AQ90 High Fidelity DNA Polymerase Master Mix

AQ90 High Fidelity DNA Polymerase 2x Master Mix is a convenient alternative to AQ90 High Fidelity DNA Polymerase, displaying the following features; high fidelity - measured up to 50x Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets.

AQ90 High Fidelity DNA Polymerase 2x Master Mix is recommended for applications, which require extremely high fidelity such as cloning/Sub-cloning, NGS applications and mutagenesis.


FEATURES

  • All-in-one 2x master mix for great convenience
  • Timesaving reaction set-up
  • High fidelity – measured up to 50x Taq Fidelity
  • 3’→5’ proofreading exonuclease activity
  • Exceptional low error rate
  • Processes up to 8.5 kb gDNA and ≤ 12kb for λDNA
  • Good coverage on DNA templates with low to high GC content


DESCRIPTION

AQ90 High Fidelity DNA Polymerase 2x Master Mix is a ready to use 2x reaction mix composed of AQ90 High Fidelity DNA Polymerase and an optimised buffer system including dNTPs and magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets.

AQ90 High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.

FIDELITY

The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerases error rate.  Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.

For more information about fidelity read here.

 

AQ90 2X MASTER MIX PROMOTES ROBUST AMPLIFICATION

AQ90 High Fidelity DNA Polymerase and AQ90 High Fidelity 2x Master Mix promotes specific and clear results on a variety of DNA targets with low to high GC content. Here we have tested 9 different DNA targets varying in both length; ranging from 200 bp to above 700 bp and in GC content; ranging from 29 – 78 %. Amplification of targets with 71 – 78 % GC content was only successful when Betaine Enhancer Solution (2 M) was added to the PCR reaction.

 

LONG RANGE AMPLIFICATION

AQ90 High Fidelity DNA Polymerase 2x Master Mix enables amplification of large and complex amplicons. Four different targets of human genomic DNA ranging from 2 kb and up to 8.5 kb was used in this study. Amplicon sizes are indicated at the top of the gel. Marker M is High Range DNA Ladder from Ampliqon (A610141)

Product info

AQ90 High Fidelity DNA Polymerase Master Mix

2x and 2.0 mM MgCl2
Sample

 

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