TEMPase Hot Start DNA Polymerase has been designed to diminish the formation of non-specific priming events during reaction set-up and the first ramp of thermal cycling. TEMPase Hot Start DNA Polymerase therefore enables detection of low abundance targets as well as multiplexing purposes.
TEMPase Hot Start DNA Polymerase is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature.
During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase. Resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase.
TEMPase Hot Start DNA Polymerase is suitable for detection of low abundance targets, screening, multiplexing, direct colony PCR and Real time PCR applications.
TEMPASE HOT START DNA POLYMERASE PROMOTES INCREASED SPECIFICITY AND YIELD
TEMPASE HOT START DNA POLYMERASE IS INACTIVE AT AMBIENT TEMPERATURE
Ampliqon TEMPase is activated by initial heating at 95 °C for 15 minutes. (lane 1). Without activation the enzyme is completely inactive (lane 2). M: marker.
TEMPase Hot Start DNA Polymerase