Hot Start

Overview

TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase activated by heat treatment. A chemical moiety is attached to the enzyme, which makes the enzyme inactive at room temperature.

During setup and the first ramp of cycling the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase. Resulting in higher specificity, increased sensitivity and greater yield compared to standard DNA polymerases.

TEMPase Hot Start DNA polymerase is manufactured in-house in accordance to our ISO 9001:2008 quality management system, ensuring that each batch possess the same robust performance every time.

TEMPase Hot Start DNA polymerase is available in different standalone variants and master mixes.

Standalone variants are supplied either without buffers or in kits including MgCl2 and one or two Ampliqon PCR buffers to achieve highest PCR performance and to avoid tedious reaction optimisation.

Master mixes include all components required for high performance PCR, just add your specific primers and DNA template.

Chemical inactivation of TEMPase Hot Start DNA Polymerase has proven highly effective compared to other inactivation methods such as antibody inactivation. The chemically modified enzyme withstands longer periods of time at room temperature without non-specific PCR amplifications. This feature is useful if you need pre-incubation at elevated temperatures, for example in case of UNG treatment at 50 °C prior to PCR.

 

Need help choosing a PCR enzyme product, getting prices, placing an order or tracking an order?

Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com