Taq DNA Polymerase Glycerol Free is a glycerol free formulation of our regular Taq DNA Polymerase and is well suited for automated high throughput testing, lyophilization or where accurate pipetting of small volumes are crucial. Taq DNA Polymerase Glycerol Free is available in different sizes and concentrations:
5 U/µl
50 U/µl
100 U/µl
FEATURES
Glycerol free storage buffer
Highly concentrated formats (50 U/µl & 100 U/µl)
Ideal for PCR kit manufactures
Processes up to 5 kb
dUTP incorporation possible
Leaves a 3'dA overhang
APPLICATIONS
Lyophilization
Automated high throughput testing
Suitable for robot-aided pipetting
DNA target detection
DESCRIPTION
Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5'→3'dA DNA polymerase and a 5'→3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability.
Highly concentrated Taq DNA Polymerase Glycerol Free is ideal for PCR kit manufactures and DNA testing facilities, requiring large amounts of DNA polymerases. The highly concentrated Taq DNA Polymerase Glycerol Free 50 U/µl & 100 U/µl allows for concentrated bulk production of PCR master mixes. Concentrated bulks in glycerol free formats are attractive when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial. Furthermore, concentrated master mixes are easier to lyophilize due to the reduced volume of the concentrated mix. Lyophilized amplification mixtures are often included in commercial PCR kits for diagnostic purposes, such as detection of pathogens, diseases and gene expression assays.
ROBOT-AIDED PIPETTING
Ampliqon Taq DNA Polymerase Glycerol Free 50U/µl is ideal for PCR Kit manufactures and DNA testing facilities.
HIGH STABILITY OF TAQ DNA POLYMERASE GLYCEROL FREE
A freeze-thaw study of Taq DNA Polymerase Glycerol Free was performed by applying 50 freeze-thaw cycles. The polymerase activity after 40 and 50 freeze-thaw cycles, respectively, was measured using real-time PCR amplification and compared to a standard serie. Initially, one sample was kept at -20 °C without any freeze-thawing to serve as reference for 100 % activity and to prepare the standard series. The amplification plots for samples representing of freeze-thaw cycles; 0, 40 and 50 is shown. The bars depict the estimated activity of Taq DNA Polymerase Glycerol Free after 0, 40 and 50 freeze-thaw cycles, respectively. Even after 50 cycles the polymerase retained 90 % of its activity. The estimated activity after 40 freeze-thaw cycles was slightly below 100 % activity, indicating just a minor decrease in activity. These results clearly show that Taq DNA Polymerase Glycerol Free is highly stable.
