PCR Buffers

4x GC Buffer I & 4x GC Buffer II

GC Buffer I and GC Buffer II is developed to promote excellent amplification in combination with TEMPase Hot Start DNA Polymerase of targets with varying degrees of GC content.


FEATURES

  • Promotes amplification of GC-rich DNA
  • 4x PCR buffer
  • Promotes diminished formation of non-specific PCR products

 
DESCRIPTION

Ammonium Buffer is often sufficient to promote amplification on most DNA targets. If Ammonium Buffer fails to promote amplification on GC-rich or difficult DNA templates we recommend to change to GC Buffer I in combination with TEMPase Hot Start DNA Polymerase. If your amplification is still not acceptable, then switch to GC Buffer II. 

TIP - WHEN TO CHOOSE SPECIALISED GC BUFFERS

If your PCR fails with TEMPase Hot Start Polymerase in combination with Ammonium Buffer, we kindly recommend you to try TEMPase in combination with GC Buffer I either as master mix or GC-rich DNA Target Kit.  If your amplification is still not satisfactory, then switch to GC Buffer II.
To save time both GC buffers can be tested at the same time.


TIP - CHOOSE THE RIGHT BUFFER

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.

Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

 

OPTIMISATION OF GC-RICH DNA AMPLIFICATION

Six genes with varying percentage of GC contents were amplified with Standard Buffer (lanes S), Ammonium Buffer (lanes A), GC Buffer I (lanes I) and GC Buffer II (lanes II).M. Marker. With an increasing percentage of GC content in the expected amplicon, Standard Buffer and Ammonium Buffer fail to support the correct amplification products, while GC Buffer I and GC Buffer II succed. Correct amplified products are encircled.

Product info

4x GC Buffer I & 4x GC Buffer II

4x GC Buffer I
4x GC Buffer II

 

Need help choosing a PCR enzyme product, getting prices, placing an order or tracking an order?

Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com