PCR Buffers

Ammonium Buffer

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.


FEATURES

  • Promotes amplification on most DNA templates
  • High yield
  • High specificity
  • Tolerance for primer annealing temperature
  • 10x Buffer


AVAILABLE IN 4 FORMULATIONS:

  • 15 mM MgCl2,
  • Mg2+ free
  • Tween free
  • Mg2+ + Tween free


DESCRIPTION

Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg2+ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences. 


TIP - CHOOSE THE RIGHT BUFFER

Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM

Ammonium Buffer: Minimal need for optimisation.
A broad range of Mg2+ concentrations and temperatures results in specific products with high yield (Lanes 1.5 - 2.5 and lanes 57-66)

 

Standard Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products. (Lanes 1.5 - 2.0 and lanes 60-66)

 

Combination Buffer: Optimisation is needed.
A narrow range of Mg2+ concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg2+ concentrations or temperatures. The first part shows a Mg2+ dilution series from 0.5 - 4.5 mM MgCl2, at 60°C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl2. M: marker.

 

Product info

Ammonium Buffer

15 mM MgCl2
Mg2+ free
Tween free
Mg2+ free, Tween free

 

Need help choosing a PCR enzyme product, getting prices, placing an order or tracking an order?

Call +45 70 20 11 69 or send an email to enzyme@ampliqon.com