The master mix is based on AQ97 Hot Start High Fidelity DNA Polymerase, which is a thermostable, chimeric DNA polymerase created specifically for low-bias, high fidelity amplification of a vast range of amplicons.
The antibody-mediated inactivation of the polymerase and exonuclease functions increases specificity of the master mix, as well as enabling room temperature setup.
AQ97 HiFi Hot Start 2x Master Mix is recommended for applications, which require extremely high fidelity such as cloning/sub-cloning, NGS applications, SNP analysis and mutagenesis.
AQ97 HiFi Hot Start 2x Master Mix is a ready to use 2x reaction mix composed of AQ97 Hot Start High Fidelity DNA Polymerase and an optimized buffer system including dNTPs and magnesium chloride, allowing robust amplification of DNA targets with low to high GC content and long DNA targets.
AQ97 Hot Start High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity, enabling accurate correction of base-pair mismatches. The attached DNA-binding domain enhances processivity, while the antibody-mediated inactivation ensures specificity even at room temperature setup. Together, these features yield an enzyme that consistently delivers low-error, specific, and fast results with long-range capacity and low-bias amplification.
For targets longer than 11 kb we recommend the stand-alone AQ97 Hot Start High Fidelity DNA Polymerase. The ability to adjust reaction conditions, such as dNTPs and enzyme concentration, enables it to amplify DNA targets up to 18 kb.
Addition of 1-2 M Betaine Enhancer Solutions is recommended when amplifying difficult amplicons, such as GC-rich targets, long targets, or those with complex secondary structures.
FIDELITY
Polymerase fidelity refers to the accuracy with which a DNA polymerase synthesizes a complementary strand during replication. The fidelity rate is influenced by factors such as the specific polymerase used, the buffer system, and the quality of the template DNA.
For more information about fidelity read here.
The fidelity of AQ97 High Fidelity DNA Polymerase was examined with a novel NGS-based method, counting the number of base substitutions within a PCR product. Data is available here.
