10x Combination Buffer

10x Combination Buffer is a balanced ammonium-potassium PCR buffer. Combination Buffer results in tolerance towards optimization of primer annealing temperatures and Mg²⁺ concentrations.

Features

Promotes reliable amplification on most DNA templates
Tolerance toward primer annealing temperature
10x buffer

DESCRIPTION

AVAILABLE IN 4 FORMULATIONS

10x Ammonium Buffer is recommended for most PCR applications however; we recommend that you continue using 10x Standard Buffer if you have already optimized your protocols for this buffer. The use of 10x Standard buffer most often requires optimization of primer annealing temperatures and Mg²⁺ concentrations. Highly pure DNA templates are preferable if you use this buffer.

15 mM MgCl_2,
Mg²⁺ free
Tween free
Mg²⁺free + Tween free

TIP - CHOOSE THE RIGHT BUFFER

Mg²⁺ free buffer is recommended if you need to optimize Mg²⁺concentrations in your PCR set-up, especially if your application requires Mg²⁺concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

Ammonium Buffer:
Minimal need for optimization.
A broad range of Mg²⁺concentrations and temperatures results in specific products with high yield (Lanes 1.5 - 2.5 and lanes 57-66)

Standard Buffer:
Optimization is needed.
A narrow range of Mg²⁺concentrations and temperatures results in specific products. (Lanes 1.5 - 2.0 and lanes 60-66)

Combination Buffer:
Optimization is needed.
A narrow range of Mg²⁺concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg²⁺ concentrations or temperatures. The first part shows a Mg²⁺ dilution series from 0.5 - 4.5 mM MgCl₂ at 60 °C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl₂. M: marker.