10x Combination Buffer is a balanced ammonium-potassium PCR buffer. Combination Buffer results in tolerance towards optimization of primer annealing temperatures and Mg2+ concentrations.
AVAILABLE IN 4 FORMULATIONS:
10x Ammonium Buffer is recommended for most PCR applications however; we recommend you to use 10x Combination Buffer as it show good results on certain PCR instruments. 10x Combination Buffer is also worth testing when searching for PCR buffers to be used in new set-up.
TIP - CHOOSE THE RIGHT BUFFER
10x Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.
Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.
Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.
PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM
Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg2+ concentrations or temperatures. The first part shows a Mg2+ dilution series from 0.5 - 4.5 mM MgCl2, at 60°C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl2. M: marker.