One-step PCR Clean-up for optimal sequencing results. Clean-up results in 5 minutes.
FEATURES
DESCRIPTION
PureIT ExoZAP PCR CleanUp consists of a balanced combination of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP).
Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp, removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp directly to the reaction containing the amplified PCR product. After treatment at 37 °C for minimum 2 minutes, PureIT ExoZAP PCR CleanUp is completely inactivated by heating at 80 °C for minimum 3 minutes.
PROTOCOL
This protocol serves as a guideline for clean-up of 5 μl PCR product using PureIT ExoZAP PCR CleanUp*.
*If treating PCR product of higher volume, then increase proportionally the amount of PureIT ExoZAP PCR CleanUp.
**PureIT ExoZAP PCR CleanUp works in PCR buffers.
PureIT ExoZAP PCR CleanUp IMPROVES QUALITY OF DNA SEQUENCING
No sample loss after treatment with PureIT ExoZAP PCR CleanUp.
Product info
PureIT ExoZAP PCR CleanUp
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