G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes.
G2 DNA/RNA Enhancer is available freeze dried with 0.1 mm or 1.4 mm beads in a 2 ml tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500 µl.
G2 DNA/RNA Enhancer should be used in combination with either a standardized extraction methods or commercial kits intended for DNA & RNA extraction.
Unsure which bead size is right for your application? We recommend testing both 0.1 mm and 1.4 mm beads to determine which provides the best results for your specific requirements.
To help you decide, we offer samples that include both sizes, allowing you to make an informed choice.
In low biomass clay sub-soils the addition of G2 DNA/RNA Enhancer has a large effect on DNA yield. Using a commercial kit, intended for extraction of DNA from soil, the increase in DNA yield with G2 DNA/RNA Enhancer was up to 10-fold. Samples was tested by 8 different laboratories. 25 samples with and without G2 DNA/RNA Enhancer beads were extracted in each laboratory. All the samples were analysed using rpoB gene copy number. The triplicates DNA extracts were tested for significance using T-test.