Q-Extract DNA Extraction Solution

The performance of Q-Extract DNA Extraction Solution was compared to fast DNA extraction protocols from competitor L and B. DNA was extracted from 3 mg of chicken muscle tissue using the respective extractions solutions and the recommended protocols. A four-fold serial dilution of the extracted DNA was amplified using Taq DNA Polymerase 2x Master Mix RED. The results indicate that the quality and yield of DNA extract using Q-Extract DNA Extraction Solution is slightly better than that of competitor L, but lower than competitor B. The protocols for Q-Extract DNA Extraction Solution and competitor L are similar. Both protocols provide reliable PCR-ready DNA in 9 to10 minutes. Competitor B provides DNA with higher yield, but the protocol from supplier B is less user-friendly, takes double time and requires more handling. M: DNA marker Iqon Low DNA Ladder. Lane 1-7: Chicken HRTP1 (245 bp).

Q-Extract DNA Extraction Solution was used to extract the PCR-ready DNA from leaves of stinging nettle and ivy. To examine the abillity of the PCR-ready DNA extracts to be used for real-time PCR applications the extracts were amplified using primers targeting chloroplast DNA (trnL 72 bp). For this experiment 5-fold serial dilutions of the extracted DNA were prepared. 7 dilutions (0.2 µl/ reaction) all in dublicate were included in the experiment.

Data showing log amplification plots of 5-fold serial dilutions of stinging nettle and ivy all in dublicates. 7 dilutions (0.2 µl of each dilution/reaction) of each dublicate were included. A: Amplification plots of DNA extracted from stining nettle using Q-Extract DNA Extraction Solution. B. Amplification plots of DNA extracted from ivy using Q-Extract DNA Extraction Solution. Cycling was performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) with the following protocol: 95 °C, 15 min; followed by 40 cycles of 95 °C, 15 sec; 60 °C, 60 sec.