Q-Extract DNA Extraction PCR Kit

The Q-Extract DNA Extraction PCR Kit is ideal for genotyping. It offers 8-minute DNA extraction protocol followed by amplification using Taq DNA Polymerase 2x Master Mix RED.

Features

Fast 8-minute extraction protocol with minimal handling

Genotyping of various matrices

PCR-ready DNA

Direct gel loading

Scalable setup

Automation-friendly

DESCRIPTION

The Q-Extract DNA Extraction PCR Kit consists of Q-Extract DNA extraction solution and Taq DNA Polymerase 2x Master Mix RED.

The non-toxic Q-Extract DNA Extraction Solution is optimized for easy extraction of PCR-ready DNA. DNA can be extracted from various mammalian tissues e.g. mouse tails, ear snips, liver, kidney, lung and plant leaves and saliva. The one-step lysis is performed in ether a thermocycler or heating block and is divided into two simple heating steps.

The extracted DNA is ready for PCR and real-time PCR without further handling such as vortex, centrifugation or dilutions. DNA extraction from fish fins and bird feathers is similar but requires prolonged incubation for optimal lysis.

The extracted DNA is amplified using the user-friendly Taq DNA Polymerase 2x Master Mix RED for PCR.

Taq DNA Polymerase 2x Master Mix RED is a ready-to-use reaction solution. The red dye and stabilizer within the master mix formulation enables the user to load amplified PCR samples directly to the DNA gel. The red dye also provides visualization of pipetting and mixing

The obtained DNA extract is stable at -20 °C for up to one week and at -80 °C for long term storage.

The Q-Extract DNA Extraction Solution is also available in these formats:

Find more information about Taq DNA Polymerase 2x Master Mix RED:

Taq DNA Polymerase 2x Master Mix RED

EFFICIENT AMPLIFICATION OF DNA EXTRACTED FROM VARIOUS MAMMALIAN TISSUES

Q-Extract DNA Extraction PCR Kit was used to extract and amplify genomic DNA from various mammalian tissues. For DNA extraction, 0.5 -10 mg tissue or 20 µl salvia was added to100 µl of Q-Extract DNA Extraction Solution. M: DNA marker Iqon Low DNA Ladder. Lane 1-5: Different mouse tissues as depicted, GADPH (266 bp). Lane 6: Chicken muscle tissue, HRPT1 (245 bp) and Lane 7: Human saliva, DMD17 (415 bp).

PERFORMANCE OF Q-EXTRACT DNA EXTRACTION KIT COMPARED TO TWO EQUIVALENT PCR KITS FOR GENOTYPING

The performance of Q-Extract DNA Extraction PCR Kit was compared to the genotyping PCR kits from competitor B and K. DNA extracts were extracted from chicken muscle (lanes 1-2) and mouse tail (lanes 3-4). Each extraction and amplification have been conducted according to the supplier manuals and using the respective DNA extractions reagents and PCR master mixes. The result shows that the Q-Extract DNA Extraction PCR Kit performs equally well or better than competitor B and K. M: Iqon Low DNA Ladder. Lane 1: Chicken HRTP1 (245 bp), Lane 2: Chicken GADPH (775 bp), Lane 3: Mouse GADPH (265 bp) and Lane 4: Mouse B-actin (318 bp)

REAL-TIME PCR ON PLANT DNA EXTRACTED BY Q-EXTRACT DNA EXTRACTION SOLUTION

Real Time PCR Amplification Curves Q-Extract

Q-Extract DNA Extraction Solution was used to extract the PCR-ready DNA from leaves of stinging nettle and ivy. To examine the abillity of the PCR-ready DNA extracts to be used for real-time PCR applications the extracts were amplified using primers targeting chloroplast DNA (trnL 72 bp). For this experiment 5-fold serial dilutions of the extracted DNA were prepared. 7 dilutions (0.2 µl/ reaction) all in dublicate were included in the experiment.

Data showing log amplification plots of 5-fold serial dilutions of stinging nettle and ivy all in dublicates. 7 dilutions (0.2 µl of each dilution/reaction) of each dublicate were included. A: Amplification plots of DNA extracted from stining nettle using Q-Extract DNA Extraction Solution. B. Amplification plots of DNA extracted from ivy using Q-Extract DNA Extraction Solution. Cycling was performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems) with the following protocol: 95 °C, 15 min; followed by 40 cycles of 95 °C, 15 sec; 60 °C, 60 sec.