MgCl2
PCR ENZYMES - PCR Buffers

Magnesium Chloride

Mg2+is required for polymerase activity. Low Mg2+ concentrations increase the fidelity but with too low Mg2+ concentrations the polymerase will not work.

Features

  • Intended for optimization of PCR

The Mg2+ concentration available in the reaction is dependent on several parameters e.g. the presence of chelators or the dNTP concentration. Therefore, the Mg2+ concentration should be optimized.

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