Dansk

T_m Calculator

PRODUCT LINE

PRODUCT

PRIMER CONCENTRATION ?

µM

ADDITIONAL Mg²⁺ ?

mM

Primer Sequences

Primer 1 5'→3' % GC · nt

Primer 2 5'→3' % GC · nt

Results

T_m primer 1 — °C

T_m primer 2 — °C

T_a annealing — °C

Single Pair Mode: Evaluate the melting point and recommended annealing temperature for an individual primer pair.
Batch Mode: Submit a set of primer pairs to calculate melting points and annealing temperatures for all of them at once.
The method is the nearest-neighbor thermodynamic model with empirically optimized parameters.
T_m values are calculated assuming standard PCR conditions.  
Optimal annealing temperatures may require empirical optimization depending on template, primer design, and reaction setup.