PCR ENZYMES - GC-rich DNA Amplification

GC-rich DNA Target Kit

GC-rich DNA Target kit is specially designed for the amplification of GC rich DNA sequences.

Features

High success rate with amplification of GC-rich DNA
High sensitivity, specificity and product yield
Diminished formation of non-specific products
Reaction setup at room temperature

DESCRIPTION

TIP - WHEN TO CHOOSE SPECIALIZED GC BUFFERS

GC-rich DNA Target Kit offers considerable flexibility and can be used as an initial testing kit before you use one of our two GC TEMPase 2x master mixes.

The GC-rich DNA Target Kit consists of TEMPase Hot Start DNA Polymerase, 4x GC Buffer I, 4x GC Buffer II and MgCl₂, all in separate tubes. MgCl₂ is enclosed in the GC-rich DNA Target Kit, to ensure convenient optimisation.

4x GC Buffer I and II are also available separately.

If your PCR fails with TEMPase Hot Start Polymerase in combination with Ammonium Buffer, we kindly recommend you to try TEMPase in combination with GC Buffer I either as master mix or GC-rich DNA Target Kit. If your amplification is still not satisfactory, then switch to GC Buffer II.

To save time both GC buffers can be tested at the same time.

OPTIMIZATION OF GC-RICH DNA AMPLIFICATION

OPTIMISATION OF GC RICH DNA AMPLIFICATION

Six genes with varying percentage of GC contents were amplified with Standard Buffer (lanes S), Ammonium Buffer (lanes A), GC Buffer I (lanes I) and GC Buffer II (lanes II). M. Marker. With an increasing percentage of GC content in the expected amplicon, Standard Buffer and Ammonium Buffer fail to support the correct amplification products, while GC Buffer I and GC Buffer II succeed. Correct amplified products are encircled.

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Real-time PCR

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GC-rich DNA Target Kit

Features

DESCRIPTION

TIP - WHEN TO CHOOSE SPECIALIZED GC BUFFERS

OPTIMIZATION OF GC-RICH DNA AMPLIFICATION