PCR ENZYMES - PCR Buffers

4x GC Buffer I & 4x GC Buffer II

4x GC Buffer I and 4x GC Buffer II is developed to promote excellent amplification in combination with TEMPase Hot Start DNA Polymerase of targets with varying degrees of GC content.

Features

Promotes amplification of GC-rich DNA
4x PCR buffer
Promotes diminished formation of non-specific PCR products

How to buy Distributor

DESCRIPTION

Ammonium Buffer is often sufficient to promote amplification on most DNA targets. If Ammonium Buffer fails to promote amplification on GC-rich or difficult DNA templates we recommend to change to GC Buffer I in combination with TEMPase Hot Start DNA Polymerase. If your amplification is still not acceptable, then switch to 4x GC Buffer II.

TIP - CHOOSE THE RIGHT BUFFER

TIP - WHEN TO CHOOSE SPECIALIZED GC BUFFERS

10x Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimizes the need for optimization of Mg²⁺ concentrations or the annealing temperatures.

Mg²⁺ free buffer is recommended if you need to optimize Mg²⁺concentrations in your PCR set-up, especially if your application requires Mg²⁺concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

If your PCR fails with TEMPase Hot Start Polymerase in combination with Ammonium Buffer, we kindly recommend you to try TEMPase Hot Start DNA Polymerase in combination with GC Buffer I. If your amplification is still not satisfactory, then switch to GC Buffer II.
To save time both GC buffers can be tested at the same time.

OPTIMIZATION OF GC-RICH DNA AMPLIFICATION

OPTIMISATION OF GC RICH DNA AMPLIFICATION

Six genes with varying percentage of GC contents were amplified with Standard Buffer (lanes S), Ammonium Buffer (lanes A), GC Buffer I (lanes I) and GC Buffer II (lanes II).M. Marker. With an increasing percentage of GC content in the expected amplicon, Standard Buffer and Ammonium Buffer fail to support the correct amplification products, while GC Buffer I and GC Buffer II succed. Correct amplified products are encircled.