GC TEMPase 2x Master Mix I or II are ready-to-use mixes based on either GC Buffer I or GC Buffer II. GC TEMPase master mixes are specifically developed for the amplification of GC-rich DNA sequences.
The GC TEMPase 2x master mixes consist of TEMPase Hot Start DNA Polymerase combined with either GC Buffer I or GC Buffer II, dNTPs and MgCl2. Just add DNA template and primers to successfully carry out PCR.
4x GC Buffer I and II are also available separately.
TIP - WHICH MASTER MIX TO CHOOSE FOR GC-RICH AMPLIFICATION
If your PCR fails with TEMPase Hot Start Polymerase 2x MASTER MIX A, we kindly recommend you to try GC TEMPase 2x Master Mix I. If your amplification is still not satisfactory, then switch to GC TEMPase 2x Master Mix II.
OPTIMISATION OF GC-RICH DNA AMPLIFICATION
COVERAGE STUDY OF GC TEMPASE 2x MASTER MIX I
GC TEMPase 2x master mixes