TEMPase Hot Start 2x Master Mix BLUE

TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE is a time saving alternative to TEMPase Hot Start DNA Polymerase 2x Master Mix. The blue loading dye facilitates direct gel loading. You do not need a separate loading buffer and time-consuming sample preparation before electrophoresis. This makes TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE especially suitable for high throughput testing.

TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE is a ready-to-use 2x master mix composed of dNTPs, a blue loading dye, a stabilizer, MgCl₂ and either TEMPase Buffer C (a balanced KCl / (NH₄)₂ SO₄ buffer system)  or TEMPase Buffer A (a (NH₄)₂ SO₄ buffer system).

The blue dye and stabilizer do not interfere with the PCR. If necessary, the blue dye can be removed by spin column purification or other methods. Furthermore, PCR products generated using TEMPase Hot Start DNA Polymerase 2x Master Mix BLUE, can directly be used for sequencing after treatment with either spin columns or PureIT ExoZAP PCR CleanUp.