PCR ENZYMES - PCR Buffers

10x Ammonium Buffer

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimizes the need for optimization of Mg²⁺ concentrations or the annealing temperatures.

Features

Promotes amplification on most DNA templates
High yield and specificity
Tolerance for primer annealing temperature
10x buffer

How to buy Distributor

DESCRIPTION

AVAILABLE IN 4 FORMULATIONS

Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg²⁺ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences.

15 mM MgCl_2,
Mg²⁺ free
Tween free
Mg²⁺free + Tween free

TIP - CHOOSE THE RIGHT BUFFER

Mg²⁺ free buffer is recommended if you need to optimize Mg²⁺concentrations in your PCR set-up, especially if your application requires Mg²⁺concentration lower than 1.5 mM.

Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

Ammonium Buffer:
Minimal need for optimization.
A broad range of Mg²⁺concentrations and temperatures results in specific products with high yield (Lanes 1.5 - 2.5 and lanes 57-66)

Standard Buffer:
Optimization is needed.
A narrow range of Mg²⁺concentrations and temperatures results in specific products. (Lanes 1.5 - 2.0 and lanes 60-66)

Combination Buffer:
Optimization is needed.
A narrow range of Mg²⁺concentrations and temperatures results in specific products with high yields. (Lane 1.5 and lanes 60-66)

Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg²⁺ concentrations or temperatures. The first part shows a Mg²⁺ dilution series from 0.5 - 4.5 mM MgCl₂ at 60 °C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl₂. M: marker.