PCR ENZYMES - PCR Buffers

5x PCR Buffer RED

5x PCR Buffer RED is a ready-to-use PCR buffer, including all components for standard PCR applications.

Features

All-in-one buffer for highest convenience
Direct gel loading onto agarose gels
Red colour enables easy visualization of pipetting and loading
Dye front runs at 1000 - 2000 bp on DNA 0.5 - 1.5 % agarose
Same specifications as 10x Ammonium Buffer
5x buffer

DESCRIPTION

PROMOTES SPECIFIC AND CLEAR RESULTS

5x PCR Buffer RED offers the same specifications as 10x Ammonium Buffer. It results in high yield of PCR products and minimizes the need for optimization of Mg²⁺ concentrations or the annealing temperatures. The included red dye and density reagent, eliminates the need for loading dye as well as the time consuming sample preparation before electrophoresis.

5x PCR Buffer RED promotes specific and clear results. The same DNA target was evaluated using the same PCR set-up in duplicates amplified by either Ampliqon Taq DNA Polymerase or TEMPase Hot Start DNA Polymerase.

DIRECT GEL LOADING

After end of amplification PCR products can be loaded directly onto agarose and SDS DNA gels. The red dye provides easy visualization of both pipetting and gel loading. The red dye front runs at 1000 - 2000 bp on 0.5 - 1.5 % agarose gels.

PCR ENZYMES

Endpoint PCR

Real-time PCR

PCR Accessories

PCR Related Products

OEM Solutions

CUSTOM MADE LABORATORY REAGENTS

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5x PCR Buffer RED

Features

DESCRIPTION

PROMOTES SPECIFIC AND CLEAR RESULTS

DIRECT GEL LOADING