10x Standard Buffer is the traditional potassium buffer and promotes high specificity.
AVAILABLE IN 4 FORMULATIONS:
10x Ammonium Buffer is recommended for most PCR applications however; we recommend that you continue using 10x Standard Buffer if you have already optimised your protocols for this buffer. The use of 10x Standard buffer most often requires optimisation of primer annealing temperatures and Mg2+ concentrations. Highly pure DNA templates are preferable if you use this buffer.
TIP - CHOOSE THE RIGHT BUFFER
10x Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.
Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.
Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.
PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM
Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg2+ concentrations or temperatures. The first part shows a Mg2+ dilution series from 0.5 - 4.5 mM MgCl2, at 60°C. The second part shows a temperature gradient from 51 - 66 °C at 1.5 mM MgCl2. M: marker.