RealQ Plus 2x Master Mix for Probe enables real-time-based DNA amplification with high specificity and efficiency. Real-time PCR is a sensitive and reliable method for gene analysis and DNA quantification. RealQ Plus 2x Master Mix for Probe is the right choice, when expenses and experiment preparation time might be limited or if you need to quickly analyse many genes.
RealQ Plus 2x Master Mix for Probe is composed of TEMPase Hot Start DNA Polymerase, dNTPs, dye and an optimised buffer system. Furthermore, this master mix is available with high, low or without ROX™ ensuring optimal performance on most of the commonly used real-time PCR instruments. Just add DNA template, primers and probe.
The RealQ Plus 2x Master Mix for probe is optimised to TaqMan probes, but is also suitable with other probe chemistries such as Molecular Beacon and Scorpion.
Most probe-based detection methods take advantage of fluorescent resonance energy transfer (FRET) by quenching the signal of a fluorescent reporter in the absence of the desired target. During elongation, the quenching molecule is separated from the fluorescent reporter and a signal is emitted and monitored. Probe-based detection is significantly more specific since signal is only detected when the correct target is amplified.
SELECT THE CORRECT ROX LEVEL
Use the Real-time PCR instrument guide to find the correct level of ROX™ reference dye matching your Real-time PCR instrument.
FAST REAL-TIME PROTOCOLS
The RealQ Plus master mixes have been tested with fast protocols with run time < 50 minutes. The application note can be found here.
HIGH EFFICIENCY AND PRECISION
STABILITY AT ROOM TEMPERATURE
RealQ Plus 2x Master Mix for Probe