Taq DNA Polymerase

Ampliqon Taq DNA Polymerase was compared with a Taq DNA Polymerase from a well-recognised competitor. Three different DNA target varying in length PAH (203 bp), ENG9 (293 bp) and  ENG 5 (480 bp) were evaluated. Ampliqon Taq DNA Polymerase performed equally well or better in this study. Furthermore, Ampliqon Taq DNA Polymerase displays a higher specificity on the PAH and ENG5 targets, than the competing Taq DNA Polymerase. Each Amplification has been conducted according to supplier's manual.

Amplification performance on five different DNA target increasing in length (788 bp – 5223 bp) were evaluated using Taq DNA Polymerase. For DNA targets exceeding 3000 bp (CFTR, AT-OUT, and PDK1), the amplification performance was optimized by employing 35 cycles and extending the elongation time. Additionally, the inclusion of 5% DMSO in the PCR reaction setup was necessary for the amplification of the longest DNA target, PDK1 (5223 bp). All five DNA targets tested in this study were successfully amplified using Taq DNA Polymerase. This study demonstrates that Ampliqon Taq DNA Polymerases can effectively amplify DNA targets of up to at least 5000 base pairs (bp).