Q Extract Dna Extraction Hot Start Pcr Kit
PCR ENZYMES - Genotyping

Q-Extract DNA Extraction Hot Start PCR Kit

The Q-Extract DNA Extraction PCR Kit is ideal for genotyping. It offers 8-minute DNA extraction protocol followed by amplification using TEMPase Hot Start DNA Polymerase 2x Master Mix A BLUE.

Features

  • Fast 8-minute extraction protocol with minimal handling
  • Genotyping of various matrices
  • PCR-ready DNA
  • Direct gel loading
  • Scalable setup
  • Automation-friendly

DESCRIPTION

The Q-Extract DNA Extraction Hot Start PCR Kit consists of Q-Extract DNA Extraction Solution and TEMPase Hot Start DNA Polymerase 2x Master Mix A BLUE.

The non-toxic Q-Extract DNA Extraction Solution is optimized for easy extraction of PCR-ready DNA. DNA can be extracted from various mammalian tissues e.g., mouse tails, ear snips, liver, kidney, lung, plant leaves and saliva.  The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps.

The extracted DNA is ready for PCR and real-time PCR without further handling such as vortex, centrifugation or dilutions. DNA extraction from fish fins and bird feathers is similar but requires prolonged incubation for optimal lysis.

The extracted DNA is amplified using the user-friendly TEMPase Hot Start DNA Polymerase 2x Master Mix A BLUE for PCR.

TEMPase Hot Start DNA Polymerase 2x Master Mix A BLUE is a ready-to-use reaction solution. The blue dye and stabilizer within the master mix formulation enables the user to load amplified PCR samples directly to the DNA gel. The red dye also provides visualization of pipetting and mixing

The obtained DNA extract is stable at -20 °C for up to one week and at -80 °C for long term storage.


The Q-Extract DNA Extraction Solution is also available in other formats:


Find more information about DNA Polymerase TEMPase Hot Start DNA Polymerase 2x Master Mix A BLUE

PERFORMANCE OF Q-EXTRACT DNA EXTRACTION HOT START PCR KIT


The performance of Q-Extract DNA Extraction Hot Start PCR Kit  (lanes 1-4) was compared to the performance of Q-Extract DNA Extraction PCR Kit (lanes 5-8). DNA extracts were extracted from mouse liver tissue. The result shows that the Q-Extract DNA Extraction Hot Start PCR Kit provides high PCR yield and more specific amplification especially on the mouse β-aktin target (absence of primer dimers). Lanes 1,2:5 and 6: Mouse GADPH (265 bp) and lanes 3,4,7 and 8: Mouse β-aktin (318 bp).

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