PCR ENZYMES - Real-time qPCR

RealQ Plus 2x Master Mix Green

RealQ Plus 2x Master Mix Green enables real-time-based DNA amplification with high specificity and efficiency.

Features

Reliable master mix for real-time PCR
Based on DNA-binding fluorescent dye detection
Reliable quantification
High specificity, stability, reproducibility and efficiency
Reaction setup at room temperature
Premixed all-in-one 2x solution

DESCRIPTION

RealQ Plus 2x Master Mix Green is composed of TEMPase Hot Start DNA Polymerase, dNTPs, fluorescent dye and an optimized buffer system. Furthermore, this master mix is available with high, low or without ROXensuring optimal performance on most of the commonly used real-time PCR instruments. Just add DNA template and primers.

When the fluorescent Green dye is free in the solution, it emits a very low fluorescent signal. As soon as the dye binds to the double-stranded DNA the signal increases significantly (thousand fold), which makes the fluorescent signal of the dye directly proportional to the amount of amplified dsDNA.

HIGH EFFICIENCY AND SPECIFICITY

To examine the efficiency of the RealQ Plus 2x Master Mix Green, High ROX, a 4-fold dilution series with human gDNA was set up for PAH target (203 bp). Samples were made in triplicates starting with 80 ng down to 80 pg gDNA per well. The results depicted here shows high precision and efficiencies close to 100 %. The identical melt curves show a high specificity of the product and the standard curve shows linear detection range and a high accuracy of the TEMPase Hot Start DNA Polymerase.

STABILITY AT ROOM TEMPERATURE

Two plates were pre-assembled for qPCR reaction and incubated in darkness at room temperature for 48 and 72 hours. The results show high stability and complete inactivation of the TEMPase before hot start. It allows the scientist to set up the reaction and run the plate several hours later, when convenient.

SELECT THE CORRECT ROX LEVEL

FAST REAL-TIME PROTOCOLS

Use the Real-time PCR instrument guide to find the correct level of ROX reference dye matching your Real-time PCR instrument.

The RealQ Plus 2x master mixes have been tested with fast protocols with run time < 50 minutes. The application note can be found here.

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Endpoint PCR

Real-time PCR

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