PCR ENZYMES - Taq DNA Polymerases

Taq DNA Polymerase Master Mix RED

Taq DNA Polymerase Master Mix RED is a very popular alternative to Taq DNA Polymerase Master Mix with the same advantages. The inert red dye and stabilizer are extra features, which allow direct loading onto your agarose gel for analysis.

Features

Ready-to-use 2x Taq master mix
Direct loading onto agarose
Red dye visualization of pipetting, mixing, and loading
Increased reproducibility

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DESCRIPTION

Taq DNA Polymerase Master Mix RED is a ready to use 2x master mix. Taq DNA Polymerase Master Mix RED is available in two final MgCl₂concentrations: 1.5 mM and 2.0 mM. Taq DNA Polymerase Master Mix RED is composed of Ampliqon Taq DNA Polymerase, the NH₄⁺ buffer system, dNTPs and magnesium chloride.

The front of red tracking dye runs at 300 - 1000 bp on 0.5 - 1.5 % agarose gel.

The red tracking dye and the stabilizer do not interfere with the PCR. If necessary, the red dye can be removed from the PCR product by spin column purification or other methods. Furthermore, PCR products generated using Taq DNA Polymerase Master Mix RED DNA, can directly be used for sequencing after treatment with either spin columns or PureIT ExoZAP PCR CleanUp.

COMPARISON OF TAQ DNA POLYMERASE 2x MASTER MIX RED WITH THREE COMPETITORS

Taq DNA Polymerase Master Mix RED was compared to equivalent Taq DNA Polymerase master mixes with Green dye from three leading competitors; T, K, and P. Six different DNA target varying in length BAIP (788), Q8 (727 bp), CFTR (613 bp), ENG5 (293 bp), ENG9 (293 bp), and ( PAH (203 bp) were evaluated. Ampliqon Taq DNA Polymerase Master Mix RED performed equally well or better than the three equivalent Taq DNA Polymerase master mixes with green color on the six primer targets tested, in this setup. Furthermore, Taq Master Mix RED supports amplification with high yield on the GC-rich ENG5 target in contradiction to the green master mixes from competitor T and P, which do not amplify the ENG5 DNA target at all. Each Amplification has been conducted according to suppliers manual.

DIRECT GEL LOADING

RED DYE EXHIBITS NO PCR INTERFERENCE

After end of amplification PCR products can be loaded directly onto agarose and SDS DNA gels. The red dye provides easy visualization of both pipetting and gel loading. The red dye front runs at 300 - 1000 bp on 0.5 - 1.5 % agarose gels.

The red dye does not interfere with PCR performance. Two different DNA targets; PAH (203 bp) and BAIP (788 bp) were amplified in duplicates using Taq DNA Polymerase 2x Master Mix RED and visualized on a agarose gel.

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