Taq OptiMix CLEAR 2x Master Mix is an optimised format of Taq DNA Polymerase 2x Master Mix and is therefore a good alternative to Taq DNA Polymerase and Taq DNA Polymerase 2x Master Mix. Taq OptiMix CLEAR 2x Master Mix ensures the user an increased specificity and improved PCR performance, even on difficult templates. Taq OptiMix CLEAR 2x Master Mix offers reduced set-up time and eliminates the risk of contamination of reaction components. Taq OptiMix CLEAR 2x Master Mix is suitable for standard PCR applications, screening and high throughput testing.
FEATURES
All-in-one 2x master mix for great convenience
Timesaving reaction setup
Increased specificity compared to Taq DNA Polymerase Master Mix
dUTP incorporation possible
Processes up to 3 kb
Leaves a 3'dA overhang
DESCRIPTION
Taq OptiMix CLEAR is a ready to use 2x reaction mix. Taq OptiMix CLEAR 2x Master Mix is composed of Ampliqon Taq DNA polymerase and an optimised buffer system including dNTPs and magnesium chloride
COMPARISON BETWEEN TAQ OPTIMIX CLEAR 2x MASTER MIX AND THREE COMPETITORS
Taq OptiMix CLEAR was compared with corresponding Taq DNA Polymerase master mixes from three well recognised competitors; T,K and N. Six different primer targets (BAIP, Q8, CFTR, ENG5, ENG9 and PAH) were evaluated. Taq OptMix CLEAR performed equally well or better on all six primer targets tested in this set up.
Each amplification was conducted according to supplier’s manual.
