The RealQ Fast 2x Master Mix Green offers a high-quality, convenient solution for dye-based real-time PCR workflows. With its optimized formulation, this ready-to-use master mix ensures sensitive, reliable, and rapid results, facilitated by the inclusion of 2Taq Hot Start DNA Polymerase.
The key to RealQ Fast's performance lies in the antibody-mediated hot-start mechanism of 2Taq, providing precise control over enzyme activity. This feature prevents undesirable amplification at low temperatures, minimizing the risk of nonspecific amplification.
For real-time PCR instruments requiring a reference dye such as ROX, the separate ROX solution is available, allowing for adjustment of ROX levels. This adaptability ensures compatibility with a wide range of real-time PCR instruments, making RealQ Fast a versatile choice for researchers.
For detailed guidelines on optimizing ROX levels to your specific real-time PCR instrument, please refer to the documentation available at ampliqon.com.
SENSITIVE DETECTION AND ACCURATE QUANTIFICATION
The RealQ Fast 2x Master Mix Green provides sensitive detection and quantification across a wide range of DNA templates including human genomic DNA, plasmid DNA and bacterial DNA.
ROOM TEMPERATURE STABILITY
The incorporation of an antibody-mediated hot-start mechanism in the RealQ Fast 2x Master Mix enables users to prepare reactions up to 72 hours before PCR cycling, without compromising performance. This feature not only offers convenience but also underscores the stability and reliability of the master mix. RealQ Fast provides users with a high level of flexibility, instilling confidence in its performance across a wide range of workflows.
REDUCED RUN TIME
RealQ Fast Green 2x Master Mix has been optimized to deliver sensitive detection and accurate quantification even when a low run time is required. RealQ Fast offers a high degree of flexibility in PCR program design, while maintaining efficiency, high specificity, and high sensitivity*.
*For set-ups demanding a low LOD, it is recommended to utilize the Standard or Fast programs.